8 Jul 2016 The Ku protein, a heterodimer of Ku70 and Ku80, binds to chromosomal replication origins maximally at G1-phase and plays an essential role
No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Ku70/80 mediates a recognition of DNA breaks to initiate nonhomologous end joining, an important DNA repair mechanism for maintaining genomic integrity. Our study describes dynamic movements of the S The Ku protein has a critical function in the repair of double-strand DNA The minimal functional interaction domain of Ku80 that interacted with Ku70 was 1 Jun 2018 The Ku70/80 heterodimer (also named Lupus Ku autoantigen protein p70 The Ku70/80 has a role in double-stranded break repair pathway, 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and 5 Dec 2016 to be critical in hepatitis B, Ku70/80 sensing of HBV DNA likely plays a critical role in immune cell recruitment in response to HBV infection. 8 Jul 2016 The Ku protein, a heterodimer of Ku70 and Ku80, binds to chromosomal replication origins maximally at G1-phase and plays an essential role Ku70 and Ku80 form a heterodimer called Ku that is well known for its role in repairing DNA double-strand breaks by nonhomologous end joining (NHEJ) (33). The The molecular function of Pso2p in DNA repair is unknown, but yeast and In addition, Ku70 and Ku80 null animals have growth defects and premature 5 Dec 2006 DNA-PK consists of the DNA end-binding Ku70/80 heterodimer and the DNA-PK The adaptor function of XRCC4 would also explain why the 21 Jun 2001 component, a heterodimer of two proteins, Ku70 and Ku80, and a catalytic subunit, DNA-PKcs. The main function of the.
ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). 1999-01-01 Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples. Supplied as 500 µL purified antibody (0.2 mg/mL). The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends.
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sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions. The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5.
20 Jul 2019 the regulation of DNA non-homologous end-joining through Ku70/80 As PAXX is a Ku interactor with a role in NHEJ, the effect of PAXX
Five of these, K539 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and … 2013-02-06 The Ku70 protein is involved in numerous cell functions, the nonhomologous end joining (NHEJ) DNA repair pathway being the best known. Here, we report a novel function for this protein in the grasshopper Eyprepocnemis plorans . Ku70/Ku80 Antibodies The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5.
av J Fransson · 2006 — Functionally-associated target antigens in cancer. id: 5decc108-1738-478b-80d5-d92c6f2f70a5 (old id 547281); date added to LUP: 2016-04-04 12:19:11
is feasible, with CDK12 absence possibly signifying defective DDR function. reduced expression of a number of DDR proteins including ATR, Ku70/Ku80,
PCR förstärka en kb 5 'uppströms sekvens av Ku70 genen med glycerol till en slutlig koncentration av 25% (v / v) och förvara vid -80 ° C för långtidsförvaring. Analysis of regions essential for the function of chromosomal
exempelvis Rad51, DNA-PK, Ku70/80, DNA-ligas IV, BRCA1 och BRCA2.
and the limited processing of your personal data in order to function. Regulation of telomere length by fatty acid elongase 3 in yeast: Involvement of inositol phosphate metabolism and Ku70/80 function Suriyan Ponnusamy, Nathan L. Alderson, Hiroko Hama, Jacek Bielawski, James C. Jiang, Rashna Bhandari, Solomon H. Snyder , S. Michal Jazwinski, Besim Ogretmen Ku70 functions in addition to nonhomologous end joining in pancreatic β-Cells. Omid Tavana, Nahum Puebla-Osorio, Jiseong Kim, Mei Sang, Stella Jang, Chengming Zhu. Ku70, to discover the effect of cellular responses to DNA damage in pancreatic β-cells on … Abstract In this study, the ku70 and ku80 homologs from the Aspergillus niger genome were identiﬁed and their function was analyzed using targeted mutagenesis. The role of the ku80 gene in non-homologous end-joining (NHEJ) was investigated by calculating the frequency of homolo-gous recombination.
As DNA end joining would be detrimental to chromosome ends, it was though that Ku has to be kept away from telomeres to prevent deleterious telomere-telomere fusion. This study highlights Ku70 as an important player not only in maintaining genomic stability through NHEJ-dependent functions, but also in regulating pancreatic β-cell proliferation, a novel NHEJ-independent function. Indeed, Ku70/80 has been proposed to perform at least two functions at telomeres.
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2007-01-23 · Function; Names & Taxonomy Ku80 and XRCC6/Ku70. KYSGRDSLIF LVDASKAMFE 60 70 80 90 100 SQSEDELTPF DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK 110
Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains . KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation 2012-02-10 · Our study shows that the addition of Ku70/80 blocks Exo1-mediated DNA end resection of the forked dsDNA substrate.